Practical work

You will be able to choose your practical work sessions only after you have paid the registration fee and passed the laboratory safety test.

You will receive an email with access details for the e-learning platform, where you can complete the laboratory safety test and select your practical sessions. The selection will open on October 15, 2025.

Your practical work preferences will be considered until all available places are filled. Sessions with a limited number of participants will be allocated on a first-come, first-served basis. Please make sure to rank all practical work sessions by priority, as it is possible that your second or third choice will be assigned if your first choice is full.

The selection period will close on November 2, 2025. Participants who do not submit their preferences by this date will be assigned to the remaining available sessions.

Laboratory safety test

You will receive an email with access details for the e-learning platform, where you can complete the laboratory safety test and select your practical sessions.

If you do not receive the email with access details by October 15, 2025, please contact as soon as possible.

List of Practical Work Sessions

Below you can find a list of this year practical work sessions. We are offering on-site practical work sessions as well as interactive online training sessions for those who are participating EBTT online.

Laboratory exercises

Practical work session	On-site	On-line
L1 – Gene electrotransfer using locally enhanced electric field
L2 – Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM
L3 – Visualization of local ablation zone distribution between two needle electrodes
L4 – Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experiments
L5 – Triggering action potential and electroporation in excitable cells exposed to electric pulses
L6 – E. coli inactivation by pulsed electric fields in a continuous mode
L7 – Influence of the orientation of the electric field on the formation of the DNA membrane complex
L8 – Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
L9 – Influence of the orientation of the electric field on gene electrotransfer efficiency and cell viability
L10 – Monitoring of electric field distribution in biological tissue by means of magnetic resonance electrical impedance tomography
L11 – Techniques of detecting and characterizing electroporation in plant tissues
L12 – Modelling, visualizing, and tracking pH front formation in tissue phantoms
L13 – Simultaneous measurement of sarcomere shortening and calcium transients in primary rat cardiomyocytes exposed to electrical pulses
L14 – Visualizing gas bubble formation during pulsed field delivery
L15 – Visualization of cytoskeletal filaments with super-resolution structured illumination microscopy (SIM)
L16 – Electroporation for treatment of cardiac arrhythmias

Computer modelling

Practical work session	On-site	On-line
C1 – Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position
C2 – Numerical modeling of thermal effects during irreversible electroporation treatments
C3 – Molecular dynamics simulations of membrane electroporation

Hardware development and measurement

Practical work session	On-site	On-line
H1 – Measurement of electroporation pulses with an oscilloscope, voltage, and current probes
H2 – Development of pulsed power generators for electroporation