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Labwork

Practical work

You will be able to choose practical work sessions only after you have paid the registration fee and have passed the laboratory safety test. Your chosen practical work preferences will be taken into account, until the places for practical work sessions are filled. Sessions with a limited number of participants will be filled on a first-come-first-served basis. Please be sure to mark all practical work sessions by priority, as practical work sessions have limited number of participants, therefore it’s possible that your second or third choice will be considered instead of your first one.


Laboratory safety test

You will be able to take laboratory safety test and select practical work sessions in lms.ebtt.org e-learning platform after registration. You should receive an e-mail with instructions how to access the e-learning platform.

S1 – Laboratory safety

S2 – Electroporation hardware safety

List of laboratory exercises

Below you can find a list of this year practical work sessions. We are offering on-site practical work sessions as well as interactive online training sessions for those who are participating EBTT online.

Laboratory exercises

Labwork On-site On-line
  L1 – Gene electrotransfer using locally enhanced electric field    
  L2 – Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM    
  L3 – Visualization of local ablation zone distribution between two needle electrodes    
  L4 – Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experiments    
  L5 – Triggering action potential and electroporation in excitable cells exposed to electric pulses    
  L6 – E. coli inactivation by pulsed electric fields in a continuous mode    
  L7 – Influence of the orientation of the electric field on the formation of the DNA membrane complex    
  L8 – Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS    
  L9 – Influence of the orientation of the electric field on gene electrotransfer efficiency and cell viability    
  L10 – Monitoring of electric field distribution in biological tissue by means of magnetic resonance electrical impedance tomography    
  L11 – Techniques of detecting and characterizing electroporation in plant tissues    
  L12 – Modelling, visualizing, and tracking pH front formation in tissue phantoms    
  L13 – Simultaneous measurement of sarcomere shortening and calcium transients in primary rat cardiomyocytes exposed to electrical pulses    
  L14 – Visualizing gas bubble formation during pulsed field delivery    
  L15 – Visualization of cytoskeletal filaments with super-resolution structured illumination microscopy (SIM)    
  L16 – Electroporation for treatment of cardiac arrhythmias    

Computer modelling

Labwork On-site On-line
  C1 – Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position    
  C2 – Numerical modeling of thermal effects during irreversible electroporation treatments    
  C3 – Molecular dynamics simulations of membrane electroporation    

Hardware development and measurement

Labwork On-site On-line
  H1 – Measurement of electroporation pulses with an oscilloscope, voltage, and current probes    
  H2 – Development of pulsed power generators for electroporation