Programme
Programme
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Schedule last updated: 4 June, 2025
Highlights of 2024
Electrophysiological effects of electroporation on myocardium, Quim Castellvi
Irreversible electroporation as an ablation technique, Rafael Davalos
Electrochemotherapy as salvage therapy for vertebral metastases, Frederic Deschamps
Collateral effects of electroporation (heating, pain&contraction, electrochemistry), Antoni Ivorra
The origin of bubbles in PFA, Samo Mahnič-Kalamiza
The role of IRE in interventional radiology and oncology, Martijn Meijerink
Bleomycin ElectroctroSclerotherapy (BEST) in treatment of vascular malformations, Tobian Muir
PEF in food processing, Indrawati Oey
My experience with new PFA technology, Helmut Pürerfellner
Preliminary programme
Electrical properties of cells and tissues in electric field
- Resting and induced transmembrane voltage
- Dielectric properties of biological tissues
- Temperature and conductivity changes in tissues
Physical chemistry of membrane electroporation
- Electrochemical membrane thermodynamics
- Molecular dynamics simulations
Electroporation in vitro and in vivo – protocols
- Electroporation of cells in vitro
- Electroporation of cells in vivo
Development of devices and electrodes
- Devices for in vitro and in vivo experimental work
- Electrodes for in vitro and in vivo
- Electroporation of cells in tissue – models of electroporation in vivo and their experimental validations
Electrochemotherapy of tumors
- Basic mechanisms of electrochemotherapy
- Preclinical studies
- Clinical use of electrochemotherapy
- Current developments and future perspectives
Application of electroporation in gene transfection
- Gene transfection in vitro
- Gene transfection in vivo
- Electroporation for gene therapy
- Electroporation for DNA vaccine
Laboratory and practical work
More details on the Labwork page
- L1 Gene electrotransfer using locally enhanced electric field
- L2 Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM
- L3 Visualization of local ablation zone distribution between two needle electrodes
- L4 Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experiments
- L5 Triggering action potential and electroporation in excitable cells exposed to electric pulses
- L6 E. coli inactivation by pulsed electric fields in a continuous mode
- L7 Analysis of electric field orientations on gene electrotransfer – visualization at the membrane level
- L8 Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
- L9 Analysis of electric field orientations on gene electrotransfer efficiency
- L10 Monitoring of electric field distribution in biological tissue by means of magnetic resonance electrical impedance tomography
- L11 Techniques of detecting and characterising electroporation in plant tissues
- L12 Modelling, visualising, and tracking pH front formation in tissue phantoms
- L13 Simultaneous measurement of sarcomere shortening and calcium transients in primary rat cardiomyocytes exposed to electroporation
- L14 Visualising gas bubble formation during pulsed field delivery
- C1 Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position
- C2 Numerical modeling of thermal effects during irreversible electroporation treatments
- C3 Molecular dynamics simulations of membrane electroporation
- H1 Measurement of electroporation pulses with oscilloscope, and voltage and current probes
- H2 Development of pulsed power generators for electroporation